Application of PCR for Selection of Gram-Positive Bacteria with High DNA G+C Content among New Isolates.
نویسندگان
چکیده
منابع مشابه
New vector for efficient allelic replacement in naturally nontransformable, low-GC-content, gram-positive bacteria.
A shuttle vector designated pMAD was constructed for quickly generating gene inactivation mutants in naturally nontransformable gram-positive bacteria. This vector allows, on X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) plates, a quick colorimetric blue-white discrimination of bacteria which have lost the plasmid, greatly facilitating clone identification during mutagenesis. The ...
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The mechanism of DNA replication is one of the driving forces of genome evolution. Bacterial DNA polymerase III, the primary complex of DNA replication, consists of PolC and DnaE. PolC is conserved in Gram-positive bacteria, especially in the Firmicutes with low GC content, whereas DnaE is widely conserved in most Gram-negative and Gram-positive bacteria. PolC contains two domains, the 3'-5'exo...
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15 صفحه اولNew thermosensitive plasmid for gram-positive bacteria.
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Small regulatory RNAs (sRNAs) that act by base-pairing were first discovered in so-called accessory DNA elements--plasmids, phages, and transposons--where they control replication, maintenance, and transposition. Since 2001, a huge body of work has been performed to predict and identify sRNAs in a multitude of bacterial genomes. The majority of chromosome-encoded sRNAs have been investigated in...
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ژورنال
عنوان ژورنال: Actinomycetologica
سال: 2002
ISSN: 1881-6371,0914-5818
DOI: 10.3209/saj.16_1